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Shanghai GenePharma
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Elabscience Biotechnology
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Enzo Biochem
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GenScript corporation
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Charles River Laboratories
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Fisher Scientific
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Obio Technology Corp Ltd
recombinant adenovirus associated virus (raav)-enhanced green fluorescent protein (egfp) constructs containing full-length human 14-3-3ε ![]() Recombinant Adenovirus Associated Virus (Raav) Enhanced Green Fluorescent Protein (Egfp) Constructs Containing Full Length Human 14 3 3ε, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/recombinant adenovirus associated virus (raav)-enhanced green fluorescent protein (egfp) constructs containing full-length human 14-3-3ε/product/Obio Technology Corp Ltd Average 90 stars, based on 1 article reviews
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Biomol GmbH
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Fisher Scientific
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ABclonal Biotechnology
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Becton Dickinson
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Becton Dickinson
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Image Search Results
Journal: Cellular & Molecular Biology Letters
Article Title: AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells
doi: 10.1186/s11658-024-00601-w
Figure Lengend Snippet: Extracellular 14-3-3ε and α-actinin 4 conferred the chemotaxis effect of AGR2-modulated CM. A WCL and CM samples of CF41.Mg or CMT-U27 transfected with pcDNA3.1-myc.His-AGR2 or the mock control were collected as described previously. Levels of indicated proteins in individual WCL samples were analyzed by immunoblotting. B CM of AGR-expressing CF41.Mg was deprived of 14-3-3ε or α-actinin 4 by immunoprecipitation (IP). Input CM and IP protein products were analyzed by immunoblotting. C , D CM samples before and after IP were subjected to a transwell migration assay. E CM samples of Ctrl-S3 cells grown at sub-confluency were collected and applied to IP, followed by immunoblotting analysis as described in B . F , G CM samples of Ctrl-S3 ( F ) or CMT-U27e ( G ) cells supplied with the indicated antibodies (3 μg each) were placed in the bottom well for a transwell migration assay. H CM of KO-S10 supplied with rcAGR2, rc14-3-3ε, or BSA at the indicated concentration was placed in the bottom well for a transwell migration assay. Data of all transwell migration assays were presented as the mean + SD ( n = 4), and a two-tailed unpaired t-test determined statistical significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001
Article Snippet:
Techniques: Chemotaxis Assay, Transfection, Control, Western Blot, Expressing, Immunoprecipitation, Transwell Migration Assay, Concentration Assay, Migration, Two Tailed Test
Journal: Journal of Cell Science
Article Title: The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage
doi: 10.1242/jcs.169573
Figure Lengend Snippet: Interaction between 14-3-3ε and CD13. (A) Representative SPR sensorgrams showing the binding of recombinant human 14-3-3ε (red, 10 µM), human anti-CD13 antibody SJ1D1 (blue, 1 µg/ml), and isotype IgG1 control (green, 1 µg/ml) on recombinant human CD13 immobilized on the sensor chip. The differential response was obtained by subtracting the signal in the blank channel from that in the experimental channel. Response is expressed in resonance units (RU) relative to time in seconds. (B) Kinetics of 14-3-3ε binding to CD13. Six concentrations of 14-3-3ε (5, 7.5, 10, 15 and 20 µM) were tested. The base line (in red) corresponds to HBS-EP alone. Software analysis gave a K D value of 2.45×10 −6 M with χ-squared value 2.09.
Article Snippet:
Techniques: Binding Assay, Recombinant, Software